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| Registros recuperados: 20 | |
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| Miyashiro,Simone; Scarcelli,Eliana; Piatti,Rosa M.; Campos,Fabíola R.; Vialta,Airton; Keid,Lara Borges; Dias,Ricardo Augusto; Genovez,Margareth E.. |
| A total of 192 samples of illegal cheese from different regions of the states of São Paulo and Minas Gerais, Brazil, were analyzed for the isolation and detection of Brucella spp. DNA by means of microbiological culture and polymerase chain reaction (PCR), respectively. Samples that yielded positive results were submitted to the analysis of the occurrence of Brucella abortus (biovars 1, 2 e 4), as well as to the differentiation of DNA in B19 vaccinal strain or Brucella abortus field strain using PCR. Although the microorganism was not isolated from any sample, PCR detected 37 positive samples (19.27%) using genus-specific primers. From these, all (100%) were Brucella abortus. Differentiation of the strain showed that 30/37 samples (81.08%) were vaccinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella abortus; B19; PCR; Cheese. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100005 |
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| Martínez,Rodrigo; Dunner,Susana; Toro,Rubén; Tobón,Jaime; Gallego,Jaime; Cañón,Javier. |
| The resistance/susceptibility of selected cattle breeds to brucellosis was evaluated in an F1 population generated by crossing animals classified as resistant (R) and susceptible (S) (R x R, R x S, S x R, S x S) based on challenges in vitro and in vivo. The association between single nucleotide polymorphisms identified in the coding region of the Slc11a1 gene and resistance/susceptibility was estimated. The trait resistance or susceptibility to brucellosis, evaluated by a challenge in vitro, showed a high heritable component in terms of additive genetic variance (h² = 0.54 ± 0.11). In addition, there was a significant association (p < 0.05) between the control of bacterial survival and two polymorphisms (a 3'UTR and SNP4 located in exon 10). The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blanco Orejinegro Creole breed; Brahman breed; Brucella abortus; Genetic resistance; Zebu. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300014 |
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| Silva,Teane Milagres Augusto da; Oliveira,Raquel Gonzaga de; Mol,Juliana Pinto da Silva; Xavier,Mariana Noyma; Paixão,Tatiane Alves da; Cortez,Adriana; Heinemann,Marcos Bryan; Richtzenhain,Leonardo José; Lage,Andrey Pereira; Santos,Renato de Lima. |
| Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Abortion; Brucella abortus; Salmonella sp.; Neospora caninum; Listeria monocytogenes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028 |
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| Martínez Herrera,D.I; Peniche Cardeña,A; Hernández Ruiz,S.G; Abeledo,Ma. Antonia; Barradas Piña,F.T; Villanueva Valencia,M; Morales Álvarez,J.F; Flores Castro,R. |
| Para evaluar la eficacia de la cepa S19 de Brucella abortus en hatos bovinos del municipio de Actopan, Veracruz que presentan una prevalencia baja de brucelosis, se emplearon 162 hembras procedentes de tres hatos, con prácticas de manejo y alimentación similares; estas fueron seleccionadas de manera aleatoria y divididas en dos grupos de 81 animales cada uno. Al primer grupo se les administró 2 mL de cepa S19 de Brucella abortus, con dosis clásica (5 X 10(10) Unidades Formadoras de Colonias) a las becerras de tres a seis meses de edad y con dosis reducida (3 X 10(8) a 3 X 10(9) Unidades Formadoras de Colonias), a las hembras de más de seis meses, incluso gestantes. El otro grupo se manejó como grupo control sin vacunar. Todos los animales fueron... |
| Tipo: Journal article |
Palavras-chave: Brucelosis; Brucella abortus; Cepa 19; Vacuna; Baja prevalencia. |
| Ano: 2011 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2011000100007 |
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| Bastos,Carla Resende; Mathias,Luis Antonio; Jusi,Márcia Mariza Gomes; Santos,Renata Ferreira dos; Silva,Glaucenyra Cecília Pinheiro da; André,Marcos Rogério; Machado,Rosangela Zacarias; Bürger,Karina Paes. |
| Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella abortus; Animal health; Zoonoses; 2-Mercaptoethanol; Complement fixation test. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300564 |
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| LANGONI,Helio; ICHIHARA,Sílvio Massaru; SILVA,Aristeu Vieira da; PARDO,Renata Bonini; TONIN,Flávia Bechelli; MENDONÇA,Lia Jeanne Pereira; MACHADO,José Aparecido Dorta. |
| Brucellosis is a chronic zoonosis that plays an important role in Public Health. Considering the lack of data in Brazil regarding its presence in raw milk and non-pasteurized dairy products, we determined the presence of brucellae in milk from brucellosis positive animals. The slide agglutination test (SAT), tube agglutination test (TAT) and TAT treated with 2-mercaptoethanol were used to identify positive animals in studied herds. For 3 days, 300 ml milk samples/cow (75 ml/teat) were collected from all productive quarters of the positive animals. These were mixed and centrifuged. Part of the pellet and of the supernatant were inoculated in Farrel and Brodie-Sinton (BS) media supplemented with antimicrobial agents. The inoculated plates and tubes were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Brucella abortus; Cows; Brucellosis. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600004 |
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| Aguiar,Daniel Moura de; Cavalcante,Guacyara Tenório; Vasconcellos,Silvio Arruda; Megid,Jane; Salgado,Vanessa Riesz; Cruz,Tais Fukuta; Labruna,Marcelo Bahia; Pinter,Adriano; Silva,Jean Carlos Ramos da; Moraes,Zenaide Maria; Camargo,Luís Marcelo Aranha; Gennari,Solange Maria. |
| Foram avaliados 304 cães de ambiente rural e urbano do município de Monte Negro, Rondônia, através do Antígeno Acidificado Tamponado (AAT), Soroaglutinação Lenta em Tubos (SAL) e 2-Mercaptoetanol (2-ME) para a pesquisa de anticorpos anti-Brucella abortus e da Imunodifusão em gel de ágar (IDGA) e Imunodifusão em gel de ágar com soro tratado com 2-Mercaptoetanol (IDGA-ME) para Brucella canis. Foram consideradas positivas as amostras reagentes nas provas confirmatórias do 2-ME e IDGA-ME. Verificaram-se 56 (18,4%) animais reagentes ao AAT e 12 (4,0%) reagentes a SAL. Apenas um cão (0,3%) foi considerado positivo, confirmado pela prova do 2-ME. Foram observadas 11 (3,6%) reações á IDGA, porém não houve confirmação na prova do IDGA-ME. Ressalta-se a baixa... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella abortus; Brucella canis; Cães; Amazônia; Sorologia. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000500039 |
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| Motta Giraldo,Javier Leonardo; Clavijo Hoyos,José Angel; Waltero García,Indira; Abeledo,María Antonia. |
| Con el objetivo de determinar y comparar la prevalencia de anticuerpos a Brucella abortus, Leptospira sp. y Neospora caninum, se investigaron siete predios, dos de bovinos, dos bubalinos y tres mixtos; además se incluyeron tres instalaciones para la venta de búfalos. La prevalencia de anticuerpos fue mayor en bubalinos que en bovinos para las tres entidades estudiadas, Leptospira sp. (37.3% vs 28%); Neospora caninum (45.4% vs 12.5%) y Brucella abortus (11.9% vs, 5.3%). Hubo presencia de anticuerpos a los seis serovares tanto en bovinos como en bubalinos y los valores de seroprevalencia a los serovares Grippotyphosa y Hardjo, fueron superiores en las dos especies. La prevalencia de anticuerpos para leptospirosis fue superior en los predios simples, tanto en... |
| Tipo: Journal article |
Palavras-chave: Brucella abortus; Leptospira sp.; Neospora caninum; Búfalos; Bovinos; Prevalencia. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200002 |
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| Antunes,J.M.A.P.; Allendorf,S.D.; Appolinário,C.M.; Peres,M.G.; Perotta,J. H.; Neves,T.B.; Deconto,I.; Barros Filho,I.R.; Biondo,A.W.; Megid,J.. |
| Avaliou-se a infecção por Brucella abortus em cavalos de carroça de Curitiba e São José dos Pinhais-PR. Um total de 123 amostras foi submetido ao teste do antígeno tamponado acidificado (ATA), soroaglutinação lenta em tubos (SAL) e prova do 2-mercaptoetanol (2-ME) para confirmação dos resultados. Oito (6,5%) equinos foram positivos para o ATA e um animal permaneceu positivo ao teste confirmatório. Existem evidências da presença de brucelose entre os cavalos de carroça. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cavalos de carroça; Brucelose; Brucella abortus; Zoonose. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000200044 |
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| Almeida,A.C.; Santorelli,A.; Bruzadelli,R.M.Z.; Oliveira,M.M.N.F.. |
| The prevalence of canine brucellosis was evaluated in the city of Alfenas, MG through the technique of agarose gel imunodifusion for Brucella canis and slow serum agglutination test with 2-mercaptoetanol for Brucella abortus. The prevalence was of 14.2% and 2.8%, respectively, for B. canis and B. abortus. The positives, characterized by animals above one year of age (77.8%), and mongrel dogs (56.2%), showed a prevalence of 50 and 48% for males and females, respectively. The canine brucellosis was prevalent in the city principally in dogs of outskirts. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cão; Brucella canis; Brucella abortus; Brucelose canina; Diagnóstico. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000200021 |
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| Almeida,A.C.; Meneses,A.M.; Bernis,V.M.O.; Soares,T.M.P.; Loiola,C.F.; Marinovick,C.; Ferreira,P.A.S.. |
| One hundred and two blood serum samples from dogs referred to the Veterinary Teaching Hospital of Alfenas University, were submitted to the agarose immune diffusion and to the fast serum-agglutination tests addressed to find antibodies anti- Brucella canis and B. abortus, respectively. Five samples (4.9%) were positives for B. canis and none for B. abortus. This prevalence is considered by some as an alert for an epidemiologic and public healthy problems, considering the serious zoonotic aspect of canine brucellosis |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cão; Brucelose; Brucella canis; Brucella abortus; Brucella canis; Brucella abortus. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000300014 |
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| Oliveira,S.C.; Harms,J.S.; Rech,E.L.; Rodarte,R.S.; Bocca,A.L.; Goes,A.M.; Splitter,G.A.. |
| Cellular immune responses are a critical part of the host's defense against intracellular bacterial infections. Immunity to Brucella abortus crucially depends on antigen-specific T cell-mediated activation of macrophages, which are the major effectors of cell-mediated killing of this organism. T lymphocytes that proliferate in response to B. abortus were characterized for phenotype and cytokine activity. Human, murine, and bovine T lymphocytes exhibited a type 1 cytokine profile, suggesting an analogous immune response in these different hosts. In vivo protection afforded by a particular cell type is dependent on the antigen presented and the mechanism of antigen presentation. Studies using MHC class I and class II knockout mice infected with B. abortus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: T cell subsets; Brucella abortus; Knockout mice; Cytokines; Intracellular pathogens; Genetic immunization. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100010 |
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| Dreer,Márcia Küster de Paula; Gonçalves,Daniela Dib; Caetano,Isabel Cristina da Silva; Gerônimo,Edson; Menegas,Paulo Henrique; Bergo,Danilo; Lopes-Mori,Fabiana Maria Ruiz; Benitez,Aline; Freitas,Julio Cesar de; Evers,Fernanda; Navarro,Italmar Teodorico; Martins,Lisiane de Almeida. |
| Background : Leptospirosis, toxoplasmosis and brucellosis are diseases with worldwide distribution. Among stray dogs, these zoonoses are facilitated by direct contact with other animal species, by the habit of scavenging garbage and hunting in search of food, drinking standing water, smelling other animals' urine, licking female genitalia and the sexual act itself. The objective of this study was to detect antibodies anti-Toxoplasma gondii, anti-Leptospira spp., anti-Brucella canis and anti-Brucella abortus in stray dogs housed in shelters at Umuarama city, Paraná, Brazil. In order to detect toxoplasmosis, indirect immunofluorescence assay (IFA) was performed, agglutination microscopic (MAT) test for leptospirosis and agar gel immunodiffusion (AGID) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira spp; Brucella abortus; Brucella canis; Toxoplasma gondii; Zoonosis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100313 |
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| Carrisoza-Urbina,Iván; Medina-Cruz,Mario; Palomares-Reséndiz,Erika Gabriela; Díaz-Aparicio,Efrén. |
| La transmisión de Brucella abortus a becerras de vacas positivas y negativas se determinó a la primera semana de vida y al tercer mes de edad. Se trabajó con dos hatos: el hato 1, con 670 vacas en producción, presentaba una seroprevalencia a brucelosis de 21.6% (145/670). En este hato se formaron dos grupos: vacas positivas y vacas negativas, como resultado de las pruebas de tarjeta y de inmunodifusión radial (IDR) realizadas con hapteno nativo. Se tomaron pruebas de sangre de las vacas en dos ocasiones, a la semana de edad y antes de que los animales fueran vacunados contra B. abortus. De las 22 vacas del grupo positivo, 2 (9.1%) becerras resultaron positivas a la primera semana de vida, pero no se encontraron vacas positivas a los tres meses de edad. En... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella abortus; Becerras; Inmunodifusión radial. |
| Ano: 2014 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922014000200002 |
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| Registros recuperados: 20 | |
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